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Type: Journal article
Title: Regulation of the gene encoding glutathione S-transferase M1 (GSTM1) by the Myb oncoprotein
Author: Bartley, P.
Keough, R.
Lutwyche, J.
Gonda, T.
Citation: Oncogene, 2003; 22(48):7570-7575
Publisher: Nature Publishing Group
Issue Date: 2003
ISSN: 0950-9232
Performer: School of Molecular and Biomedical Science
Statement of
Paul A Bartley, Rebecca A Keough, Jodi K Lutwyche and Thomas J Gonda
Abstract: The identification of Myb ‘target’ genes will not only aid in the understanding of how overexpression of Myb, or expression of activated forms of Myb, leads to cellular transformation but will also shed light on its role in normal cells.Using a combination of an estrogenregulated Myb-transformed cell line (ERMYB) and PCR-based subtractive hybridization, we have identified the gene (GSTM1) encoding the detoxification enzyme glutathione S-transferase M1 as being transcriptionally upregulated by Myb.Functio nal analysis of the GSTM1 promoter using reporter assays indicated that both the DNA binding and transactivation domains of Myb were required for transcriptional activation.Mutationa l analysis of consensus Myb-binding sites (MBS) in the promoter and electrophoretic mobility gel shift analysis indicated that one of the three potential MBS can bind Myb protein, and is the primary site involved in the regulation of this promoter by Myb.
Keywords: Myb; transactivation; glutathione S-transferase; promoter
Description: The definitive version can be found at
RMID: 0020030953
DOI: 10.1038/sj.onc.1207136
Appears in Collections:Medicine publications

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