Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/97544
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Type: Journal article
Title: Diversity in boron toxicity tolerance of Australian barley (Hordeum vulgare L.) genotypes
Author: Hayes, J.
Pallotta, M.
Garcia, M.
Öz, M.
Rongala, J.
Sutton, T.
Citation: BMC Plant Biology, 2015; 15(1):231-1-231-14
Publisher: BioMed Central
Issue Date: 2015
ISSN: 1471-2229
1471-2229
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Responsibility: 
Julie E. Hayes, Margaret Pallotta, Melissa Garcia, Mehmet Tufan Öz, Jay Rongala and Tim Sutton
Abstract: Boron (B) is an important micronutrient for plant growth, but is toxic when levels are too high. This commonly occurs in environments with alkaline soils and relatively low rainfall, including many of the cereal growing regions of southern Australia. Four major genetic loci controlling tolerance to high soil B have been identified in the landrace barley, Sahara 3771. Genes underlying two of the loci encode the B transporters HvBot1 and HvNIP2;1.We investigated sequence and expression level diversity in HvBot1 and HvNIP2;1 across barley germplasm, and identified five novel coding sequence alleles for HvBot1. Lines were identified containing either single or multiple copies of the Sahara HvBot1 allele. We established that only the tandemly duplicated Sahara allele conferred B tolerance, and this duplicated allele was found only in a set of nine lines accessioned in Australian collections as Sahara 3763-3771. HvNIP2;1 coding sequences were highly conserved across barley germplasm. We identified the likely causative SNP in the 5'UTR of Sahara HvNIP2;1, and propose that the creation of a small upstream open reading frame interferes with HvNIP2;1 translation in Sahara 3771. Similar to HvBot1, the tolerant HvNIP2;1 allele was unique to the Sahara barley accessions. We identified a new source of the 2H B tolerance allele controlling leaf symptom development, in the landrace Ethiopia 756.Ethiopia 756, as well as the cultivar Sloop Vic which carries both the 2H and HvBot1 B tolerance alleles derived from Sahara 3771, may be valuable as alternative parents in breeding programs targeted to high soil B environments. There is significant diversity in B toxicity tolerance among contemporary Australian barley varieties but this is not related to variation at any of the four known B tolerance loci, indicating that novel, as yet undiscovered, sources of tolerance exist.
Keywords: Chromosomes, Plant; Hordeum; Plant Leaves; Boron; Adaptation, Physiological; Gene Duplication; Alleles; Molecular Sequence Data; Genetic Variation; Genetic Loci
Rights: © 2015 Hayes et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
RMID: 0030036857
DOI: 10.1186/s12870-015-0607-1
Appears in Collections:Agriculture, Food and Wine publications

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