Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/97561
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Type: Journal article
Title: Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors
Author: Wu, L.
Russell, D.
Wong, S.
Chen, M.
Tsai, T.
St John, J.
Norman, R.
Febbraio, M.
Carroll, J.
Robker, R.
Citation: Development, 2015; 142(4):681-691
Publisher: Company of Biologists
Issue Date: 2015
ISSN: 0950-1991
1477-9129
Statement of
Responsibility: 
Linda L. Wu, Darryl L. Russell, Siew L. Wong, Miaoxin Chen, Te-Sha Tsai, Justin C. St John, Robert J. Norman, Mark A. Febbraio, John Carroll, Rebecca L. Robker
Abstract: Over-nutrition in females causes altered fetal growth during pregnancy and permanently programs the metabolism of offspring; however, the temporal and mechanistic origins of these changes, and whether they are reversible, are unknown. We now show that, in obese female mice, cumulus-oocyte complexes exhibit endoplasmic reticulum (ER) stress, high levels of intracellular lipid, spindle abnormalities and reduced PTX3 extracellular matrix protein production. Ovulated oocytes from obese mice contain normal levels of mitochondrial (mt) DNA but have reduced mitochondrial membrane potential and high levels of autophagy compared with oocytes from lean mice. After in vitro fertilization, the oocytes of obese female mice demonstrate reduced developmental potential and form blastocysts with reduced levels of mtDNA. Blastocysts transferred to normal weight surrogates that were then analyzed at E14.5 showed that oocytes from obese mice gave rise to fetuses that were heavier than controls and had reduced liver and kidney mtDNA content per cell, indicating that maternal obesity before conception had altered the transmission of mitochondria to offspring. Treatment of the obese females with the ER stress inhibitor salubrinal or the chaperone inducer BGP-15 before ovulation increased the amount of the mitochondrial replication factors TFAM and DRP1, and mtDNA content in oocytes. Salubrinal and BGP-15 also completely restored oocyte quality, embryo development and the mtDNA content of fetal tissue to levels equivalent to those derived from lean mice. These results demonstrate that obesity before conception imparts a legacy of mitochondrial loss in offspring that is caused by ER stress and is reversible during the final stages of oocyte development and maturation.
Keywords: BGP-15; ER stress; Mitochondria; mtDNA; Obesity; Ovary
Rights: © 2015. Published by The Company of Biologists Ltd
RMID: 0030022644
DOI: 10.1242/dev.114850
Grant ID: http://purl.org/au-research/grants/nhmrc/1061819
http://purl.org/au-research/grants/nhmrc/1041471
Appears in Collections:Medicine publications

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