Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/97598
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Type: Journal article
Title: Characterization and phylogenetic analysis of allergenic Tryp-alpha-amyl protein family in plants
Author: Wang, J.
Yang, L.
Zhao, X.
Li, J.
Zhang, D.
Citation: Journal of Agricultural and Food Chemistry, 2014; 62(1):270-278
Publisher: American Chemical Society
Issue Date: 2014
ISSN: 0021-8561
1520-5118
Statement of
Responsibility: 
Jing Wang, Litao Yang, Xiaoxiang Zhao, Jing Li, and Dabing Zhang
Abstract: Most known allergenic proteins in rice ( Oryza sativa ) seed belong to the Tryp_alpha_amyl family (PF00234), but the sequence characterization and the evolution of the allergenic Tryp_alpha_amyl family members in plants have not been fully investigated. In this study, two specific motifs were found besides the common alpha-amylase inhibitors (AAI) domain from the allergenic Tryp_alpha_amyl family members in rice seeds (trRSAs). To understand the evolution and functional importance of the Tryp_alpha_amy1 family and the specific motifs for the allergenic one, a BLAST search identified 75 homologous proteins of trRSAs (trHAs) from 22 plant species including main crops such as rice, maize ( Zea mays ), wheat ( Triticum aestivum ), and sorghum ( Sorghum bicolor ) from all available sequences in the public databases. Statistical analysis showed that the allergenicity of trHAs is closely associated with these two motifs with high number of cysteine residues (p value = 0.00026), and the trHAs with and without the two motifs were clustered into separate clades, respectively. Furthermore, significant difference was observed on the secondary and tertiary structures of allergenic and nonallergenic trHAs. In addition, expression analysis showed that trHA-encoding genes of purple false brome ( Brachypodium distachyon ), barrel medic ( Medicago truncatula ), rice, and sorghum are dominantly expressed in seeds. This work provides insight into the understanding of the properties of allergens in the Tryp_alpha_amyl family and is helpful for allergy therapy.
Keywords: rice seed allergen; allergenic protein; Tryp_alpha_amyl family; evolution of trHAs; allergen-specific characteristics
Description: Publication Date (Web): December 12, 2013
Rights: © 2013 American Chemical Society
RMID: 0030023433
DOI: 10.1021/jf402463w
Appears in Collections:Agriculture, Food and Wine publications

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