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Type: Journal article
Title: Osteoprotegerin expression in synovial tissue from patients with rheumatoid arthritis, spondyloarthropathies and osteoarthritis and normal controls
Author: Haynes, D.
Barg, E.
Crotti, T.
Holding, C.
Weedon, H.
Atkins, G.
Zannettino, A.
Ahern, M.
Coleman, M.
Roberts-Thomson, P.
Kraan, M.
Tak, P.
Smith, M.
Citation: Rheumatology, 2003; 42(1):123-134
Publisher: Oxford Univ Press
Issue Date: 2003
ISSN: 1462-0324
Statement of
D. R. Haynes, E. Barg, T. N. Crotti, C. Holding, H. Weedon, G. J. Atkins, A. Zannetino, M. J. Ahern, M. Coleman, P. J. Roberts-Thomson, M. Kraan, P. P. Tak, and M. D. Smith
Abstract: Objectives. To demonstrate the expression of osteoprotegerin (OPG) and receptor activator of nuclear factor B ligand (RANKL) in synovial tissue from rheumatoid arthritis (RA) patients, establish the cell lineage expressing OPG and compare the expression of OPG in RA, spondyloarthropathies, osteoarthritis and normal synovial tissue. Methods. Synovial biopsy specimens were obtained at arthroscopy from 16 RA and 12 spondyloarthropathy patients with active synovitis of a knee joint, six RA patients with no evidence of active synovitis, 10 patients with osteoarthritis and 18 normal subjects. Immunohistological analysis was performed using monoclonal antibodies (mAb) to detect OPG and RANKL expression. In addition, dual immunohistochemical evaluation was performed with lineage-specific monoclonal antibodies (macrophages, fibroblasts and endothelial cells) and OPG to determine the cell lineages expressing OPG. The sections were evaluated by computer-assisted image analysis and semiquantitative analysis. Results. Two patterns of OPG expression were seen, one exclusively in endothelial cells and one expressed predominantly in macrophages in the synovial lining layer. Both patterns of OPG staining could be blocked with excess recombinant OPG. Endothelial and synovial lining expression of OPG was seen in all synovial tissues except those from patients with active RA. In contrast, RANKL expression was seen predominantly in synovial tissue from patients with active disease, mainly in sublining regions, particularly within areas of lymphocyte infiltration. Conclusions. OPG expression on macrophage type synovial lining cells as well as endothelial cells is deficient in RA patients with active synovitis, in contrast to that seen in spondyloarthropathy patients with active synovitis. This deficiency in OPG expression in the inflamed joint of RA patients may be important in the development of radiologically defined joint erosions.
Keywords: Synovial Membrane
Knee Joint
Arthritis, Rheumatoid
Osteoarthritis, Knee
Acute Disease
Carrier Proteins
Membrane Glycoproteins
Receptors, Tumor Necrosis Factor
Receptors, Cytoplasmic and Nuclear
Blotting, Western
Statistics, Nonparametric
Case-Control Studies
Image Processing, Computer-Assisted
Middle Aged
RANK Ligand
Receptor Activator of Nuclear Factor-kappa B
Rights: © 2003 British Society for Rheumatology
DOI: 10.1093/rheumatology/keg047
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