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Type: Journal article
Title: Shigella flexneri cell-to-cell spread, and growth and inflammation in mice, is limited by the outer membrane protease IcsP
Author: Tran, E.
Attridge, S.
Teh, M.
Morona, R.
Citation: FEMS Microbiology Letters, 2015; 362(12):fnv088-1-fnv088-8
Publisher: Oxford University Press
Issue Date: 2015
ISSN: 0378-1097
Statement of
Elizabeth Ngoc Hoa Tran, Stephen R. Attridge, Min Yan Teh and Renato Morona
Abstract: The Shigella flexneri autotransporter protein IcsA is essential for intra- and intercellular spread, and icsA mutants are attenuated in several models. However, the pathogenic significance of the outer membrane protease IcsP, which orchestrates the polar distribution of IcsA on the bacterial surface, remains unclear. To further examine this point, we constructed icsP mutants in the two most commonly studied S. flexneri strains and evaluated their in vitro and in vivo performance relative to wild type. Both icsP mutants showed aberrant surface distribution of IcsA, but the in vitro consequences depended upon the cell line being used to assess bacterial motility and plaque formation. Evaluating the behaviour of the mutants in two mouse models suggested functional expression of icsP might limit bacterial persistence and the associated inflammation in host tissues, consistent with the findings in one of the three cell lines used.
Keywords: protease; bacterial virulence; cell-to-cell spread; mice inflammation; Shigella flexneri
Description: First published online: 29 May 2015
Rights: © FEMS 2015. All rights reserved.
RMID: 0030030114
DOI: 10.1093/femsle/fnv088
Appears in Collections:Molecular and Biomedical Science publications

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