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Type: Journal article
Title: Genome-wide transcriptome analysis during anthesis reveals new insights into the molecular basis of heat stress responses in tolerant and sensitive rice varieties
Author: González-Schain, N.
Dreni, L.
Lawas, L.
Galbiati, M.
Colombo, L.
Heuer, S.
Jagadish, K.
Kater, M.
Citation: Plant and Cell Physiology, 2016; 57(1):57-68
Publisher: Oxford University Press
Issue Date: 2016
ISSN: 0032-0781
Statement of
Nahuel González-Schain, Ludovico Dreni, Lovely M.F. Lawas, Massimo Galbiati, Lucia Colombo, Sigrid Heuer, Krishna S.V. Jagadish and Martin M. Kater
Abstract: Rice is one of the main food crops in the world. In the near future, yield is expected to be under pressure due to unfavorable climatic conditions, such as increasing temperatures. Therefore, improving rice germplasm in order to guarantee rice production under harsh environmental conditions is of top priority. Although many physiological studies have contributed to understanding heat responses during anthesis, the most heat-sensitive stage, molecular data are still largely lacking. In this study, an RNA-sequencing approach of heat- and control-treated reproductive tissues during anthesis was carried out using N22, one of the most heat-tolerant rice cultivars known to date. This analysis revealed that expression of genes encoding a number of transcription factor families, together with signal transduction and metabolic pathway genes, is repressed. On the other hand, expression of genes encoding heat shock factors and heat shock proteins was highly activated. Many of these genes are predominantly expressed at late stages of anther development. Further physiological experiments using heat-tolerant N22 and two sensitive cultivars suggest that reduced yield in heat-sensitive plants may be associated with poor pollen development or production in anthers prior to anthesis. In parallel, induction levels of a set of heat-responsive genes in these tissues correlated well with heat tolerance. Altogether, these findings suggest that proper expression of protective chaperones in anthers is needed before anthesis to overcome stress damage and to ensure fertilization. Genes putatively controlling this process were identified and are valuable candidates to consider for molecular breeding of highly productive heat-tolerant cultivars.
Keywords: Anthesis
Heat stress
Spikelet fertility
Rights: © The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email:
DOI: 10.1093/pcp/pcv174
Appears in Collections:Agriculture, Food and Wine publications
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