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Type: Journal article
Title: A cDNA clone encoding an IgE-binding protein from Brassica anther has significant sequence similarity to Ca²⁺-binding proteins
Other Titles: A cDNA clone encoding an IgE-binding protein from Brassica anther has significant sequence similarity to Ca(2+)-binding proteins
Author: Toriyama, K.
Okada, T.
Watanabe, M.
Ide, T.
Ashida, T.
Xu, H.
Singh, M.
Citation: Plant Molecular Biology: An International Journal on Molecular Biology, Molecular Genetics and Biochemistry, 1995; 29(6):1157-1165
Publisher: Kluwer Academic Publishers
Issue Date: 1995
ISSN: 0167-4412
Statement of
Kinya Toriyama, Takashi Okada, Masao Watanabe, Takeshi Ide, Tsuneo Ashida, Huiling Xu, Mohan B. Singh
Abstract: Thirteen cDNA clones encoding IgE-binding proteins were isolated from expression libraries of anthers of Brassica rapa L. and B. napus L. using serum IgE from a patient who was specifically allergic to Brassica pollen. These clones were divided into two groups, I and II, based on the sequence similarity. All the group I cDNAs predicted the same protein of 79 amino acids, while the group II predicted a protein of 83 amino acids with microheterogeneity. Both of the deduced amino acid sequences contained two regions with sequence similarity to Ca(2+)-binding sites of Ca(2+)-binding proteins such as calmodulin. However flanking sequences were distinct from that of calmodulin or other Ca(2+)-binding proteins. RNA-gel blot analysis showed the genes of group I and II were preferentially expressed in anthers at the later developmental stage and in mature pollen. The recombinant proteins produced in Escherichia coli was recognized in immunoblot analysis by the IgE of a Brassica pollen allergic patient, but not by the Ige of a non-allergic patient. The cDNA clones reported here, therefore, represent pollen allergens of Brassica species.
Keywords: Anther; Brassica napus; Brassica rapa; Ca²⁺-binding protein; cDNA cloning; pollen allergen
Rights: © 1995 Kluwer Academic Publishers
DOI: 10.1007/BF00020459
Appears in Collections:Agriculture, Food and Wine publications
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