Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/9798
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dc.contributor.authorTruongTran, A.en
dc.contributor.authorRuffin, R.en
dc.contributor.authorZalewski, P.en
dc.date.issued2000en
dc.identifier.citationAmerican Journal of Physiology-Lung Cellular and Molecular Physiology, 2000; 279(6 23-6):L1172-L1183en
dc.identifier.issn1040-0605en
dc.identifier.issn1522-1504en
dc.identifier.urihttp://hdl.handle.net/2440/9798-
dc.description.abstractThe respiratory epithelium is vulnerable to noxious substances, resulting in the shedding of cells and decreased protection. Zinc (Zn), an antioxidant and cytoprotectant, can suppress apoptosis in a variety of cells. Here we used the novel Zn-specific fluorophore Zinquin to visualize and quantify labile intracellular Zn in respiratory epithelial cells. Zinquin fluorescence in isolated ciliated tracheobronchial epithelial cells and intact epithelium from sheep and pigs revealed an intense fluorescence in the apical and mitochondria-rich cytoplasm below the cilia. Zinquin fluorescence was quenched by the Zn chelator N,N,N', N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN) and increased by the Zn ionophore pyrithione. We also assessed whether changes in intracellular labile Zn would influence susceptibility of these cells to apoptosis by hydrogen peroxide. Our results confirm that Zn deficiency enhanced hydrogen peroxide-induced caspase activation from 1.24 +/- 0.12 to 2.58 +/- 0.53 units. microg protein(-1). h(-1) (P </= 0.05); Zn supplementation suppressed these effects. These findings are consistent with the hypothesis that Zn protects upper respiratory epithelial cells and may have implications for human asthma where there is hypozincemia and epithelial damage.en
dc.language.isoenen
dc.publisherAmer Physiological Socen
dc.subjectBronchi; Pulmonary Alveoli; Respiratory Mucosa; Trachea; Tumor Cells, Cultured; Epithelial Cells; Animals; Sheep; Swine; Humans; Adenocarcinoma, Bronchiolo-Alveolar; Lung Neoplasms; Hydrogen Peroxide; Zinc; Ethylenediamines; Butyrates; Tosyl Compounds; Coumarins; Quinolones; Caspases; Oligopeptides; Chelating Agents; Fluorescent Dyes; Oxidants; Microscopy, Electron; Microscopy, Fluorescence; Apoptosis; Enzyme Activation; Drug Synergism; Caspase 3en
dc.titleVisualization of labile zinc and its role in apoptosis of primary airway epithelial cells and cell linesen
dc.typeJournal articleen
dc.identifier.doi10.1152/ajplung.2000.279.6.l1172en
pubs.publication-statusPublisheden
dc.identifier.orcidZalewski, P. [0000-0001-5196-2611]en
Appears in Collections:Medicine publications

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