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Type: Conference paper
Title: Localised hydrogen peroxide sensing for reproductive health
Author: Purdey, M.
Schartner, E.
Sutton-McDowall, M.
Ritter, L.
Thompson, J.
Monro, T.
Abell, A.
Citation: Proceedings of SPIE, as published in Optical Sensors, 2015 / Baldini, F., Homola, J., Lieberman, R. (ed./s), vol.9506, pp.950614-1-950614-10
Publisher: SPIE
Issue Date: 2015
Series/Report no.: Proceedings of SPIE
ISBN: 9781628416275
ISSN: 0277-786X
Conference Name: SPIE Optics + Optoelectronics (13 Apr 2015 - 16 Apr 2015 : Prague, Czech Republic)
Statement of
Malcolm S Purdey, Erik P Schartner, Melanie L Sutton-McDowall, Lesley J Ritter, Jeremy G Thompson, Tanya M Monro, and Andrew D Abell
Abstract: The production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H₂O₂) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H₂O₂ production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H₂O₂ that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H₂O₂ due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H₂O₂. Using this method, biologically relevant concentrations of H₂O₂ were detected, enabling remote sensing studies into ROS releases from embryos throughout early development.
Description: Session 10 - Chemical Sensors and Biosensors II
Rights: © 2015 SPIE
RMID: 0030031653
DOI: 10.1117/12.2178680
Appears in Collections:Medicine publications

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