Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/98015
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Purdey, M. | - |
dc.contributor.author | Schartner, E. | - |
dc.contributor.author | Sutton-McDowall, M. | - |
dc.contributor.author | Ritter, L. | - |
dc.contributor.author | Thompson, J. | - |
dc.contributor.author | Monro, T. | - |
dc.contributor.author | Abell, A. | - |
dc.contributor.editor | Baldini, F. | - |
dc.contributor.editor | Homola, J. | - |
dc.contributor.editor | Lieberman, R. | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Proceedings of SPIE, 2015 / Baldini, F., Homola, J., Lieberman, R. (ed./s), vol.9506, pp.950614-1-950614-10 | - |
dc.identifier.isbn | 9781628416275 | - |
dc.identifier.issn | 0277-786X | - |
dc.identifier.issn | 1996-756X | - |
dc.identifier.uri | http://hdl.handle.net/2440/98015 | - |
dc.description | Session 10 - Chemical Sensors and Biosensors II | - |
dc.description.abstract | The production of reactive oxygen species (ROS) is known to affect the developmental competence of embryos. Hydrogen peroxide (H₂O₂) an important reactive oxygen species, is also known to causes DNA damage and defective sperm function. Current techniques require incubating a developing embryo with an organic fluorophore which is potentially hazardous for the embryo. What we need is a localised ROS sensor which does not require fluorophores in solution and hence will allow continuous monitoring of H₂O₂ production without adversely affect the development of the embryo. Here we report studies on such a fibre-based sensor for the detection of H₂O₂ that uses a surface-bound aryl boronate fluorophore carboxyperoxyfluor-1(CPF1). Optical fibres present a unique platform due to desirable characteristics as dip sensors in biological solutions. Attempts to functionalise the fibre tips using polyelectrolyte layers and (3-aminopropyl)triethoxysilane (APTES) coatings resulted in a limited signal and poor fluorescent response to H₂O₂ due to a low tip surface density of the fluorophore. To increase the surface density, CPF1 was integrated into a polymer matrix formed on the fibre tip by a UV-catalysed polymerisation process of acrylamide onto a methacrylate silane layer. The polyacrylamide containing CPF1 gave a much higher surface density than previous surface attachment methods and the sensor was found to effectively detect H₂O₂. Using this method, biologically relevant concentrations of H₂O₂ were detected, enabling remote sensing studies into ROS releases from embryos throughout early development. | - |
dc.description.statementofresponsibility | Malcolm S Purdey, Erik P Schartner, Melanie L Sutton-McDowall, Lesley J Ritter, Jeremy G Thompson, Tanya M Monro, and Andrew D Abell | - |
dc.language.iso | en | - |
dc.publisher | SPIE | - |
dc.relation.ispartofseries | Proceedings of SPIE | - |
dc.rights | © 2015 SPIE | - |
dc.source.uri | http://dx.doi.org/10.1117/12.2178680 | - |
dc.title | Localised hydrogen peroxide sensing for reproductive health | - |
dc.type | Conference paper | - |
dc.contributor.conference | SPIE Optics + Optoelectronics (13 Apr 2015 - 16 Apr 2015 : Prague, Czech Republic) | - |
dc.identifier.doi | 10.1117/12.2178680 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Purdey, M. [0000-0002-5063-8972] | - |
dc.identifier.orcid | Schartner, E. [0000-0003-1669-4302] | - |
dc.identifier.orcid | Sutton-McDowall, M. [0000-0002-4121-0202] | - |
dc.identifier.orcid | Ritter, L. [0000-0001-5942-851X] | - |
dc.identifier.orcid | Thompson, J. [0000-0003-4941-7731] | - |
dc.identifier.orcid | Abell, A. [0000-0002-0604-2629] | - |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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hdl_98015.pdf | Published version | 767.99 kB | Adobe PDF | View/Open |
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