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Type: Journal article
Title: Satellite RNAs interfere with the function of viral RNA silencing suppressors
Author: Shen, W.
Au, P.
Shi, B.
Smith, N.
Dennis, E.
Guo, H.
Zhou, C.
Wang, M.
Citation: Frontiers in Plant Science, 2015; 6(Apr):281-1-281-12
Publisher: Frontiers Media S.A.
Issue Date: 2015
ISSN: 1664-462X
Statement of
Wan-Xia Shen, Phil Chi Khang Au, Bu-Jun Shi, Neil A. Smith, Elizabeth S. Dennis, Hui-Shan Guo, Chang-Yong Zhou, and Ming-Bo Wang
Abstract: Viral satellite RNAs (satRNAs) are small subviral RNAs and depend on the helper virus for replication and spread. satRNAs can attenuate helper virus-induced symptoms, the mechanism of which remains unclear. Here, we show that two virus-encoded suppressors of RNA silencing (VSRs), Cucumber mosaic virus (CMV) 2b and Tombusvirus P19, suppress hairpin RNA (hpRNA)-induced silencing of a β-glucuronidase (GUS) gene in Nicotiana benthamiana. This suppression can be overcome by CMV Y-satellite RNA (Y-Sat) via the Y-Sat-derived small interfering RNAs (siRNAs), which bind to the VSRs and displace the bound hpGUS-derived siRNAs. We also show that microRNA target gene expression in N. tabacum was elevated by CMV infection, presumably due to function of the 2b VSR, but this upregulation of microRNA target genes was reversed in the presence of Y-Sat. These results suggest that satRNA infection minimizes the effect of VSRs on host siRNA and microRNA-directed silencing. Our results suggest that the high abundance of satRNA-derived siRNAs contributes to symptom attenuation by binding helper virus-encoded VSRs, minimizing the capacity of the VSRs to bind host siRNA and miRNA and interfere with their function.
Keywords: virus-encoded suppressor; satellite RNA; symptom attenuation; RNA silencing; microRNA expression
Rights: © 2015 Shen, Au, Shi, Smith, Dennis, Guo, Zhou and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
RMID: 0030040669
DOI: 10.3389/fpls.2015.00281
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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