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Type: Journal article
Title: Variation for N uptake system in maize: genotypic response to N supply
Author: Garnett, T.
Plett, D.
Conn, V.
Conn, S.
Rabie, H.
Rafalski, J.
Dhugga, K.
Tester, M.
Kaiser, B.
Citation: Frontiers in Plant Science, 2015; 6(Nov):936-1-936-13
Publisher: Frontiers Media S.A.
Issue Date: 2015
ISSN: 1664-462X
Statement of
Trevor Garnett, Darren Plett, Vanessa Conn, Simon Conn, Huwaida Rabie, J. Antoni Rafalski, Kanwarpal Dhugga, Mark A. Tester and Brent N. Kaiser
Abstract: An understanding of the adaptations made by plants in their nitrogen (N) uptake systems in response to reduced N supply is important to the development of cereals with enhanced N uptake efficiency (NUpE). Twenty seven diverse genotypes of maize (Zea mays, L.) were grown in hydroponics for 3 weeks with limiting or adequate N supply. Genotypic response to N was assessed on the basis of biomass characteristics and the activities of the nitrate ([Formula: see text]) and ammonium ([Formula: see text]) high-affinity transport systems. Genotypes differed greatly for the ability to maintain biomass with reduced N. Although, the N response in underlying biomass and N transport related characteristics was less than that for biomass, there were clear relationships, most importantly, lines that maintained biomass at reduced N maintained net N uptake with no change in size of the root relative to the shoot. The root uptake capacity for both [Formula: see text] and [Formula: see text] increased with reduced N. Transcript levels of putative [Formula: see text] and [Formula: see text] transporter genes in the root tissue of a subset of the genotypes revealed that predominately ZmNRT2 transcript levels responded to N treatments. The correlation between the ratio of transcripts of ZmNRT2.2 between the two N levels and a genotype's ability to maintain biomass with reduced N suggests a role for these transporters in enhancing NUpE. The observed variation in the ability to capture N at low N provides scope for both improving NUpE in maize and also to better understand the N uptake system in cereals.
Keywords: N; NUE; Zea mays; ammonium; nitrate; nitrogen use efficiency; uptake
Rights: © 2015 Garnett, Plett, Conn, Conn, Rabie, Rafalski, Dhugga, Tester and Kaiser. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
RMID: 0030040743
DOI: 10.3389/fpls.2015.00936
Grant ID:
Appears in Collections:Agriculture, Food and Wine publications

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