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https://hdl.handle.net/2440/98128
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Type: | Journal article |
Title: | Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein |
Author: | Pahuja, P. Srinivasan, A. Puri, M. |
Citation: | Molecular and Cellular Biochemistry: an international journal for chemical biology in health and disease, 2014; 389(1):133-139 |
Publisher: | Springer US |
Issue Date: | 2014 |
ISSN: | 0300-8177 1573-4919 |
Statement of Responsibility: | Parveen Pahuja, Alagiri Srinivasan, Munish Puri |
Abstract: | X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering. |
Keywords: | Dispersity; Gel filtration chromatography; Dynamic light scattering; Crystallisation |
Description: | First online: 03 January 2014 |
Rights: | © Springer Science+Business Media New York 2014 |
DOI: | 10.1007/s11010-013-1934-x |
Published version: | http://dx.doi.org/10.1007/s11010-013-1934-x |
Appears in Collections: | Aurora harvest 7 Chemistry publications |
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