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|Title:||Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein|
|Citation:||Molecular and Cellular Biochemistry, 2014; 389(1):133-139|
|Parveen Pahuja, Alagiri Srinivasan, Munish Puri|
|Abstract:||X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.|
|Keywords:||Dispersity; Gel filtration chromatography; Dynamic light scattering; Crystallisation|
|Description:||First online: 03 January 2014|
|Rights:||© Springer Science+Business Media New York 2014|
|Appears in Collections:||Chemistry publications|
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