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Type: Journal article
Title: Addition of magnesium chloride to enhance mono-dispersity of a coiled-coil recombinant mouse macrophage protein
Author: Pahuja, P.
Srinivasan, A.
Puri, M.
Citation: Molecular and Cellular Biochemistry, 2014; 389(1):133-139
Publisher: Springer US
Issue Date: 2014
ISSN: 0300-8177
Statement of
Parveen Pahuja, Alagiri Srinivasan, Munish Puri
Abstract: X-ray crystallography for the determination of three-dimensional structures of protein macromolecules represents an important tool in function assignment of uncharacterized proteins. However, crystallisation is often difficult to achieve. A protein sample fully characterized in terms of dispersity may increase the likelihood of successful crystallisation by improving the predictability of the crystallisation process. To maximize the probability of crystallisation of a novel mouse macrophage protein (rMMP), target molecule was characterized and refined to improve monodispersity. Addition of MgCl2 at low concentrations resolves the rMMP into a monodisperse solution, and finally successful crystallization of rMMP was achieved. The effect of MgCl2 was studied using gel filtration chromatography and dynamic light scattering.
Keywords: Dispersity; Gel filtration chromatography; Dynamic light scattering; Crystallisation
Description: First online: 03 January 2014
Rights: © Springer Science+Business Media New York 2014
RMID: 0030045010
DOI: 10.1007/s11010-013-1934-x
Appears in Collections:Chemistry publications

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