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Type: Journal article
Title: Involvement of redox events in caspase activation in zinc-depleted airway epithelial cells
Author: Carter, J.
TruongTran, A.
Grosser, D.
Ho, L.
Ruffin, R.
Zalewski, P.
Citation: Biochemical and Biophysical Research Communications, 2002; 297(4):1062-1070
Publisher: Academic Press Inc
Issue Date: 2002
ISSN: 0006-291X
Statement of
Joanne E. Carter, Ai Q. Truong-Tran, Dion Grosser, Lien Ho, Richard E. Ruffin and Peter D. Zalewski
Abstract: Airway epithelial cells (AEC) contain both pro- and anti-apoptotic factors but little is known about mechanisms regulating apoptosis of these cells. In this study we have examined the localization of pro-caspase-3 and Zn2+, a cellular regulator of pro-caspase-3, in primary sheep and human AEC. Zn2+ was concentrated in both cytoplasmic vesicles and ciliary basal bodies, in the vicinity of both pro-caspase-3 and the antioxidant Cu/Zn superoxide dismutase (Cu/Zn SOD). Depletion of intracellular Zn2+ in sheep AEC, using the membrane permeant Zn2+ chelator TPEN, increased lipid peroxidation in the apical cell membranes (as assessed by immunofluorescence with anti-hydroxynonenal) as well as increasing activated pro-caspase-3 and apoptosis. There were smaller increases in caspase-2 and -6 but not other caspases. Activation of caspase-3 in TPEN-treated AEC was inhibited strongly by N-acetylcysteine and partially by vitamin C and vitamin E. These findings suggest that cytoplasmic pro-caspase-3 is positioned near the lumenal surface of AEC where it is under the influence of Zn2+ and other anti-oxidants.
Keywords: Zinc; Caspase-3; Cu/Zn SOD; N-acetylcysteine; Airway epithelium; Basal bodies; Zinquin; Lipid peroxidation; Anti-oxidant
Description: Copyright © 2002 Elsevier Science (USA). All rights reserved.
RMID: 0020020543
DOI: 10.1016/S0006-291X(02)02292-1
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Appears in Collections:Medicine publications

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