Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/98299
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Type: Journal article
Title: Genetic diversity and genome wide association study of β-glucan content in tetraploid wheat grains
Other Titles: Genetic diversity and genome wide association study of beta-glucan content in tetraploid wheat grains
Author: Marcotuli, I.
Houston, K.
Schwerdt, J.
Waugh, R.
Fincher, G.
Burton, R.
Blanco, A.
Gadaleta, A.
Citation: PLoS One, 2016; 11(4):e0152590-1-e0152590-15
Publisher: Public Library of Science
Issue Date: 2016
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Ilaria Marcotuli, Kelly Houston, Julian G. Schwerdt, Robbie Waugh, Geoffrey B. Fincher, Rachel A. Burton, Antonio Blanco, Agata Gadaleta
Abstract: Non-starch polysaccharides (NSPs) have many health benefits, including immunomodulatory activity, lowering serum cholesterol, a faecal bulking effect, enhanced absorption of certain minerals, prebiotic effects and the amelioration of type II diabetes. The principal components of the NSP in cereal grains are (1,3;1,4)-β-glucans and arabinoxylans. Although (1,3;1,4)-β-glucan (hereafter called β-glucan) is not the most representative component of wheat cell walls, it is one of the most important types of soluble fibre in terms of its proven beneficial effects on human health. In the present work we explored the genetic variability of β-glucan content in grains from a tetraploid wheat collection that had been genotyped with a 90k-iSelect array, and combined this data to carry out an association analysis. The β-glucan content, expressed as a percentage w/w of grain dry weight, ranged from 0.18% to 0.89% across the collection. Our analysis identified seven genomic regions associated with β-glucan, located on chromosomes 1A, 2A (two), 2B, 5B and 7A (two), confirming the quantitative nature of this trait. Analysis of marker trait associations (MTAs) in syntenic regions of several grass species revealed putative candidate genes that might influence β-glucan levels in the endosperm, possibly via their participation in carbon partitioning. These include the glycosyl hydrolases endo-β-(1,4)-glucanase (cellulase), β-amylase, (1,4)-β-xylan endohydrolase, xylanase inhibitor protein I, isoamylase and the glycosyl transferase starch synthase II.
Keywords: Triticum; Seeds; beta-Glucans; Plant Proteins; Genetic Variation; Genome-Wide Association Study; Tetraploidy
Rights: © 2016 Marcotuli et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030046369
DOI: 10.1371/journal.pone.0152590
Appears in Collections:Agriculture, Food and Wine publications

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