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Type: Journal article
Title: Accumulation of N-acetylglucosamine oligomers in the plant cell wall affects plant architecture in a dose-dependent and conditional manner
Author: Vanholme, B.
Vanholme, R.
Turumtay, H.
Goeminne, G.
Cesarino, I.
Goubet, F.
Morreel, K.
Rencoret, J.
Bulone, V.
Hooijmaijers, C.
De Rycke, R.
Gheysen, G.
Ralph, J.
De Block, M.
Meulewaeter, F.
Boerjan, W.
Citation: Plant Physiology, 2014; 165(1):290-308
Publisher: American Society of Plant Biologists
Issue Date: 2014
ISSN: 0032-0889
Statement of
Bartel Vanholme, Ruben Vanholme, Halbay Turumtay, Geert Goeminne, Igor Cesarino, Florence Goubet, Kris Morreel, Jorge Rencoret, Vincent Bulone, Cortwa Hooijmaijers, Riet De Rycke, Godelieve Gheysen, John Ralph, Marc De Block, Frank Meulewaeter, and Wout Boerjan
Abstract: To study the effect of short N-acetylglucosamine (GlcNAc) oligosaccharides on the physiology of plants, N-ACETYLGLUCOSAMINYLTRANSFERASE (NodC) of Azorhizobium caulinodans was expressed in Arabidopsis (Arabidopsis thaliana). The corresponding enzyme catalyzes the polymerization of GlcNAc and, accordingly, β-1,4-GlcNAc oligomers accumulated in the plant. A phenotype characterized by difficulties in developing an inflorescence stem was visible when plants were grown for several weeks under short-day conditions before transfer to long-day conditions. In addition, a positive correlation between the oligomer concentration and the penetrance of the phenotype was demonstrated. Although NodC overexpression lines produced less cell wall compared with wild-type plants under nonpermissive conditions, no indications were found for changes in the amount of the major cell wall polymers. The effect on the cell wall was reflected at the transcriptome level. In addition to genes encoding cell wall-modifying enzymes, a whole set of genes encoding membrane-coupled receptor-like kinases were differentially expressed upon GlcNAc accumulation, many of which encoded proteins with an extracellular Domain of Unknown Function26. Although stress-related genes were also differentially expressed, the observed response differed from that of a classical chitin response. This is in line with the fact that the produced chitin oligomers were too small to activate the chitin receptor-mediated signal cascade. Based on our observations, we propose a model in which the oligosaccharides modify the architecture of the cell wall by acting as competitors in carbohydrate-carbohydrate or carbohydrate-protein interactions, thereby affecting noncovalent interactions in the cell wall or at the interface between the cell wall and the plasma membrane.
Keywords: Cell Wall; Endoplasmic Reticulum; Plants, Genetically Modified; Arabidopsis; Plant Stems; Chitin; Lignin; N-Acetylglucosaminyltransferases; Acetylglucosamine; Bacterial Proteins; Arabidopsis Proteins; Down-Regulation; Gene Expression Regulation, Plant; Up-Regulation; Oxidative Stress; Phenotype; Penetrance; Stress, Mechanical; Models, Biological; Molecular Sequence Data; Plant Cells; Transcriptome; Chitinases
Rights: © 2014 American Society of Plant Biologists. All rights reserved.
RMID: 0030021016
DOI: 10.1104/pp.113.233742
Appears in Collections:Agriculture, Food and Wine publications

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