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https://hdl.handle.net/2440/98772
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Type: | Journal article |
Title: | Computational studies of the binding profile of phosphoinositide PtdIns (3,4,5) P₃ with the pleckstrin homology domain of an oomycete cellulose synthase |
Other Titles: | Computational studies of the binding profile of phosphoinositide PtdIns (3,4,5) P(3) with the pleckstrin homology domain of an oomycete cellulose synthase |
Author: | Kuang, G. Bulone, V. Tu, Y. |
Citation: | Scientific Reports, 2016; 6(1):20555-1-20555-11 |
Publisher: | Nature Publishing Group |
Issue Date: | 2016 |
ISSN: | 2045-2322 2045-2322 |
Statement of Responsibility: | Guanglin Kuang, Vincent Bulone & Yaoquan Tu |
Abstract: | Saprolegnia monoica is a model organism to investigate Saprolegnia parasitica, an important oomycete which causes considerable loss in aquaculture every year. S. monoica contains cellulose synthases vital for oomycete growth. However, the molecular mechanism of the cellulose biosynthesis process in the oomycete growth is still poorly understood. Some cellulose synthases of S. monoica, such as SmCesA2, are found to contain a plecsktrin homology (PH) domain, which is a protein module widely found in nature and known to bind to phosphoinositides, a class of signaling compounds involved in many biological processes. Understanding the molecular interactions between the PH domain and phosphoinositides would help to unravel the cellulose biosynthesis process of oomycetes. In this work, the binding profile of PtdIns (3,4,5) P₃, a typical phosphoinositide, with SmCesA2-PH was studied by molecular docking, molecular dynamics and metadynamics simulations. PtdIns (3,4,5) P₃ is found to bind at a specific site located at β1, β2 and β1-β2 loop of SmCesA2-PH. The high affinity of PtdIns (3,4,5) P₃ to SmCesA2-PH is contributed by the free phosphate groups, which have electrostatic and hydrogen-bond interactions with Lys88, Lys100 and Arg102 in the binding site. |
Keywords: | Saprolegnia Glucosyltransferases Phosphatidylinositol Phosphates Computer Simulation Pleckstrin Homology Domains |
Rights: | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
DOI: | 10.1038/srep20555 |
Grant ID: | SNIC2014-1-326 |
Published version: | http://dx.doi.org/10.1038/srep20555 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 7 |
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hdl_98772.pdf | Published version | 2.02 MB | Adobe PDF | View/Open |
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