Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/98801
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dc.contributor.authorDavey, J.-
dc.contributor.authorChouteau, M.-
dc.contributor.authorBarker, S.-
dc.contributor.authorMaroja, L.-
dc.contributor.authorBaxter, S.-
dc.contributor.authorSimpson, F.-
dc.contributor.authorMerrill, R.-
dc.contributor.authorJoron, M.-
dc.contributor.authorMallet, J.-
dc.contributor.authorDasmahapatra, K.-
dc.contributor.authorJiggins, C.-
dc.date.issued2016-
dc.identifier.citationG3: Genes, Genomes, Genetics, 2016; 6(3):695-708-
dc.identifier.issn2160-1836-
dc.identifier.issn2160-1836-
dc.identifier.urihttp://hdl.handle.net/2440/98801-
dc.description.abstractThe Heliconius butterflies are a widely studied adaptive radiation of 46 species spread across Central and South America, several of which are known to hybridize in the wild. Here, we present a substantially improved assembly of the Heliconius melpomene genome, developed using novel methods that should be applicable to improving other genome assemblies produced using short read sequencing. First, we whole-genome-sequenced a pedigree to produce a linkage map incorporating 99% of the genome. Second, we incorporated haplotype scaffolds extensively to produce a more complete haploid version of the draft genome. Third, we incorporated ∼20x coverage of Pacific Biosciences sequencing, and scaffolded the haploid genome using an assembly of this long-read sequence. These improvements result in a genome of 795 scaffolds, 275 Mb in length, with an N50 length of 2.1 Mb, an N50 number of 34, and with 99% of the genome placed, and 84% anchored on chromosomes. We use the new genome assembly to confirm that the Heliconius genome underwent 10 chromosome fusions since the split with its sister genus Eueides, over a period of about 6 million yr.-
dc.description.statementofresponsibilityJohn W. Davey, Mathieu Chouteau, Sarah L. Barker, Luana Maroja, Simon W. Baxter, Fraser Simpson, Richard M. Merrill, Mathieu Joron, James Mallet, Kanchon K. Dasmahapatra and Chris D. Jiggins-
dc.language.isoen-
dc.publisherGenetics Society of America-
dc.rights© 2016 Davey et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.-
dc.subjectHeliconius-
dc.subjectgenome assembly-
dc.subjectlinkage mapping-
dc.subjectchromosome fusions-
dc.subjectEueides-
dc.titleMajor improvements to the Heliconius melpomene genome assembly used to confirm 10 chromosome fusion events in 6 million years of butterfly evolution-
dc.typeJournal article-
dc.identifier.doi10.1534/g3.115.023655-
pubs.publication-statusPublished-
dc.identifier.orcidBaxter, S. [0000-0001-5773-6578]-
Appears in Collections:Aurora harvest 3
Genetics publications

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