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Type: Journal article
Title: Differential expression of microRNAs and potential targets under drought stress in barley
Author: Ferdous, J.
Sanchez-Ferrero, J.
Langridge, P.
Milne, L.
Chowdhury, J.
Brien, C.
Tricker, P.
Citation: Plant, Cell and Environment, 2017; 40(1):11-24
Publisher: Wiley
Issue Date: 2017
ISSN: 0140-7791
Statement of
Jannatul Ferdous, Juan Carlos Sanchez-Ferrero, Peter Langridge, Linda Milne, Jamil Chowdhury, Chris Brien, and Penny J. Tricker
Abstract: Drought is a crucial environmental constraint limiting crop production in many parts of the world. microRNA (miRNA) based gene regulation has been shown to act in several pathways, including crop response to drought stress. Sequence based profiling and computational analysis have revealed hundreds of miRNAs and their potential targets in different plant species under various stress conditions, but few have been biologically verified. In this study, eleven candidate miRNAs were tested for their expression profiles in barley. Differences in accumulation of only four miRNAs (Ath-miR169b, Osa-miR1432, Hv-miRx5 and Hv-miR166b/c) were observed between drought-treated and well-watered barley in four genotypes. miRNA targets were predicted using degradome analysis of two, different genotypes and genotype-specific target cleavage was observed. Inverse correlation of mature miRNA accumulation with miRNA target transcripts was also genotype-dependent under drought treatment. Drought-responsive miRNAs accumulated predominantly in mesophyll tissues. Our results demonstrate genotype-specific miRNA regulation under drought stress and evidence for their role in mediating expression of target genes for abiotic stress response in barley.
Keywords: Hordeum vulgare
canonical cleavage
in situ-RT-PCR
Rights: © 2016 The Authors. Plant, Cell & Environment Published by JohnWiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1111/pce.12764
Appears in Collections:Agriculture, Food and Wine publications
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