Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/99102
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Type: Journal article
Title: Nutrient variability in phloem: examining changes in K, Mg, Zn and Fe concentration during grain loading in common wheat (Triticum aestivum)
Author: Palmer, L.
Palmer, L.
Rutzke, M.
Graham, R.
Stangoulis, J.
Citation: Physiologia Plantarum, 2014; 152(4):729-737
Publisher: Wiley-Blackwell
Issue Date: 2014
ISSN: 0031-9317
1399-3054
Statement of
Responsibility: 
Lachlan J. Palmer, Lyndon T. Palmer, Michael A. Rutzke, Robin D. Graham and James C. R. Stangoulis
Abstract: In wheat, nutrients are transported to seeds via the phloem yet access to this vascular tissue for exudate collection and quantitative analysis of elemental composition is difficult. The purest phloem is collected through the use of aphid stylectomy with volumes of exudate collected normally in the range of 20-500 nl. In this work a new method using inductively coupled plasma mass spectroscopy (ICP-MS) was developed to measure the concentration of K, Mg, Zn and Fe in volumes of wheat (Triticum aestivum, genotype Samnyt 16) phloem as small as 15.5 nl. This improved method was used to observe changes in phloem nutrient concentration during the grain loading period. There were statistically significant increases in phloem Mg and Zn concentration and a significant decrease in K over the period from 1-2 days after anthesis (DAA) to 9-12 DAA. During this period, there was no statistically significant change in phloem Fe concentration.
Keywords: Animals
Aphids
Triticum
Seeds
Cereals
Potassium
Magnesium
Iron
Zinc
Biological transport
Phloem
Rights: © 2014 Scandinavian Plant Physiology Society
DOI: 10.1111/ppl.12211
Appears in Collections:Agriculture, Food and Wine publications
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