Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/99218
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Type: Journal article
Title: Peroxidase enzymes regulate collagen biosynthesis and matrix mineralization by cultured human osteoblasts
Author: DeNichilo, M.
Shoubridge, A.
Panagopoulos, V.
Liapis, V.
Zysk, A.
Zinonos, I.
Hay, S.
Atkins, G.
Findlay, D.
Evdokiou, A.
Citation: Calcified Tissue International, 2016; 98(3):294-305
Publisher: Springer
Issue Date: 2016
ISSN: 0171-967X
1432-0827
Statement of
Responsibility: 
Mark O. DeNichilo, Alexandra J. Shoubridge, Vasilios Panagopoulos, Vasilios Liapis, Aneta Zysk, Irene Zinonos, Shelley Hay, Gerald J. Atkins, David M. Findlay, Andreas Evdokiou
Abstract: The early recruitment of inflammatory cells to sites of bone fracture and trauma is a critical determinant in successful fracture healing. Released by infiltrating inflammatory cells, myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are heme-containing enzymes, whose functional involvement in bone repair has mainly been studied in the context of providing a mechanism for oxidative defense against invading microorganisms. We report here novel findings that show peroxidase enzymes have the capacity to stimulate osteoblastic cells to secrete collagen I protein and generate a mineralized extracellular matrix in vitro. Mechanistic studies conducted using cultured osteoblasts show that peroxidase enzymes stimulate collagen biosynthesis at a post-translational level in a prolyl hydroxylase-dependent manner, which does not require ascorbic acid. Our studies demonstrate that osteoblasts rapidly bind and internalize both MPO and EPO, and the catalytic activity of these peroxidase enzymes is essential to support collagen I biosynthesis and subsequent release of collagen by osteoblasts. We show that EPO is capable of regulating osteogenic gene expression and matrix mineralization in culture, suggesting that peroxidase enzymes may play an important role not only in normal bone repair, but also in the progression of pathological states where infiltrating inflammatory cells are known to deposit peroxidases.
Keywords: Peroxidase enzymes; Osteoblasts; Collagen biosynthesis; Matrix mineralization
Rights: © Springer Science+Business Media New York 2015
RMID: 0030040519
DOI: 10.1007/s00223-015-0090-6
Grant ID: http://purl.org/au-research/grants/nhmrc/627015
http://purl.org/au-research/grants/nhmrc/1050694
Appears in Collections:Medicine publications

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