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|Title:||Simultaneous conduction mapping and intracellular membrane potential recording in isolated atria|
|Citation:||Canadian Journal of Physiology and Pharmacology, 2016; 94(5):563-569|
|Publisher:||NRC Research Press (Canadian Science Publishing)|
|Melissa Neo, David G. Morris, Pawel Kuklik, Dennis H. Lau, Hany Dimitri, Wei-Wen Lim, Prashanthan Sanders, David A. Saint|
|Abstract:||We describe a novel approach for simultaneously determining regional differences in action potential (AP) morphology and tissue electrophysiological properties in isolated atria. The epicardial surface of rat atrial preparations was placed in contact with a multi-electrode array (9 × 10 silver chloride electrodes, 0.1 mm diameter and 0.1 mm pitch). A glass microelectrode (100 MΩ) was simultaneously inserted into the endocardial surface to record intracellular AP from either of 2 regions (A, B) during pacing from 2 opposite corners of the tissue. AP duration at 80% of repolarisation and its restitution curve was significantly different only in region A (p < 0.01) when AP was initiated at different stimulation sites. Alternans in AP duration and AP amplitude, and in conduction velocity were observed during 2 separate arrhythmic episodes. This approach of combining microelectrode array and intracellular membrane potential recording may provide new insights into arrhythmogenic mechanisms in animal models of cardiovascular disease.|
|Keywords:||high-density mapping; atrial action potential membrane recordings; electrophysiology; arrhythmogenic mechanisms; atrial fibrillation|
|Description:||Published on the web 18 November 2015.|
|Rights:||© Copyright 2014 – Canadian Science Publishing|
|Appears in Collections:||Medical Sciences publications|
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