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Type: Journal article
Title: High-throughput sequencing of trace quantities of soil provides reproducible and discriminative fungal DNA profiles
Author: Young, J.
Weyrich, L.
Cooper, A.
Citation: Journal of Forensic Sciences, 2016; 61(2):478-484
Publisher: Wiley
Issue Date: 2016
ISSN: 0022-1198
Statement of
Jennifer M. Young, Laura S. Weyrich, and Alan Cooper
Abstract: High-throughput sequencing (HTS) offers improved resolution between forensic soil samples by characterizing individual taxa present; however, the heterogeneous distribution of taxa in soils, and limited quantity of material available, may hinder the reliability of HTS in casework. Using HTS of the internal transcribed spacer, we examined the effect of soil mass (50, 150, and 250 mg) on fungal DNA profiles, focusing on reproducibility and discriminatory power between close proximity soils, and samples with similar textural classification. The results show that reduced soil mass had no significant effect on sample differentiation and that 150 mg soil provides the most reproducible DNA profiles across different soil types. In addition, Ascomycota was identified as a robust fungal target for forensic intelligence as this phylum was detected consistently across all samples regardless of sample quantity. Overall, this study highlights the value of trace quantities of soil for use in forensic casework.
Keywords: forensic science; DNA profiling; soil;high-throughput sequencing; fungi; internal transcribed spacer
Description: Version of Record online: 1 FEB 2016
Rights: © 2016 American Academy of Forensic Sciences
DOI: 10.1111/1556-4029.12996
Grant ID: ARC
Published version:
Appears in Collections:Aurora harvest 7
Australian Centre for Ancient DNA publications

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