Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/99331
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Type: Journal article
Title: Oyster viperin retains direct antiviral activity and its transcription occurs via a signalling pathway involving a heat-stable haemolymph protein
Author: Green, T.
Speck, P.
Geng, L.
Raftos, D.
Beard, M.
Helbig, K.
Citation: Journal of General Virology, 2015; 96(12):3587-3597
Publisher: Microbiology Society
Issue Date: 2015
ISSN: 0022-1317
1465-2099
Statement of
Responsibility: 
Timothy J. Green, Peter Speck, Lu Geng, David Raftos, Michael R. Beard and Karla J. Helbig
Abstract: Little is known about the response of non-model invertebrates, such as oysters, to viral infection. The vertebrate innate immune system detects virus-derived nucleic acids to trigger the type I interferon (IFN)-pathway, leading to the transcription of hundreds of IFN-stimulated genes (ISGs) that exert antiviral functions. Invertebrates were thought to lack the IFN-pathway based on the absence of IFN or ISGs encoded in model-invertebrate genomes. However, the oyster genome encodes many ISGs, including the well-described antiviral protein, viperin. In this study, we characterise oyster-viperin and show it localises to caveolin-1 and inhibits Dengue virus replication in a heterologous model. In a second set of experiments, we provide evidence that the hemolymph from poly(I:C)-injected oysters contains a heat-stable, protease-susceptible factor that induces hemocyte transcription of viperin mRNA in conjunction with upregulation of IFN-regulatory factor. Collectively, these results support the concept that oysters have antiviral systems that are homologous to the vertebrate IFN-pathway.
Keywords: Hemolymph; Animals; Dengue Virus; Lipids; Proteins; Antiviral Agents; Virus Replication; Signal Transduction; Gene Expression Regulation; Amino Acid Sequence; Molecular Sequence Data; Ostreidae; Caveolin 1; Hot Temperature
Rights: © 2015 The Authors
RMID: 0030037142
DOI: 10.1099/jgv.0.000300
Grant ID: http://purl.org/au-research/grants/nhmrc/1053206
Appears in Collections:Molecular and Biomedical Science publications

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