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|Title:||Fluorescent IGF-II analogues for FRET-based investigations into the binding of IGF-II to the IGF-1R|
|Author:||Cottam Jones, J.|
|Citation:||Organic and Biomolecular Chemistry, 2016; 14(9):2698-2705|
|Publisher:||Royal Society of Chemistry|
|J. M. Cottam Jones, P. W. R. Harris, D. B. Scanlon, B. E. Forbes, M. A. Brimble and A. D. Abell|
|Abstract:||The interaction of IGF-II with the insulin receptor (IR) and type 1 insulin-like growth factor receptor (IGF-1R) has recently been identified as potential therapeutic target for the treatment of cancer. Understanding the interactions of IGF-II with these receptors is required for the development of potential anticancer therapeutics. This work describes an efficient convergent synthesis of native IGF-II and two non-native IGF-II analogues with coumarin fluorescent probes incorporated at residues 19 and 28. These fluorescent analogues bind with nanomolar affinities to the IGF-1R and are suitable for use in fluorescence resonance energy transfer (FRET) studies. From these studies the F19Cou IGF-II and F28Cou IGF-II proteins were identified as good probes for investigating the binding interactions of IGF-II with the IGF-1R and its other high affinity binding partners.|
|Keywords:||Receptor, IGF Type 1; Insulin-Like Growth Factor II; Fluorescence Resonance Energy Transfer; Binding Sites; Molecular Structure; Fluorescence|
|Rights:||Copyright status unknown|
|Appears in Collections:||Chemistry publications|
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