Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/99629
Citations
Scopus Web of Science® Altmetric
?
?
Type: Journal article
Title: Genetics, transcriptional profiles, and catalytic properties of the UDP-Arabinose mutase family from barley
Author: Hsieh, Y.
Zhang, Q.
Yap, K.
Shirley, N.
Lahnstein, J.
Nelson, C.
Burton, R.
Millar, A.
Bulone, V.
Fincher, G.
Citation: Biochemistry, 2016; 55(2):322-334
Publisher: American Chemical Society
Issue Date: 2016
ISSN: 0006-2960
1520-4995
Statement of
Responsibility: 
Yves S. Y. Hsieh, Qisen Zhang, Kuok Yap, Neil J. Shirley, Jelle Lahnstein,Clark J. Nelson, Rachel A. Burton, A. Harvey Millar, Vincent Bulone, and Geoffrey B. Fincher
Abstract: Four members of the UDP-Ara mutase (UAM) gene family from barley have been isolated and characterized, and their map positions on chromosomes 2H, 3H, and 4H have been defined. When the genes are expressed in Escherichia coli, the corresponding HvUAM1, HvUAM2, and HvUAM3 proteins exhibit UAM activity, and the kinetic properties of the enzymes have been determined, including Km, Kcat, and catalytic efficiencies. However, the expressed HvUAM4 protein shows no mutase activity against UDP-Ara or against a broad range of other nucleotide sugars and related molecules. The enzymic data indicate therefore that the HvUAM4 protein may not be a mutase. However, the HvUAM4 gene is transcribed at high levels in all the barley tissues examined, and its transcript abundance is correlated with transcript levels for other genes involved in cell wall biosynthesis. The UDP-L-Arap → UDP-L-Araf reaction, which is essential for the generation of the UDP-Araf substrate for arabinoxylan, arabinogalactan protein, and pectic polysaccharide biosynthesis, is thermodynamically unfavorable and has an equilibrium constant of 0.02. Nevertheless, the incorporation of Araf residues into nascent polysaccharides clearly occurs at biologically appropriate rates. The characterization of the HvUAM genes opens the way for the manipulation of both the amounts and fine structures of heteroxylans in cereals, grasses, and other crop plants, with a view toward enhancing their value in human health and nutrition, and in renewable biofuel production.
Keywords: Gene expression regulation; plant
Rights: Copyright © 2015 American Chemical Society
RMID: 0030041416
DOI: 10.1021/acs.biochem.5b01055
Grant ID: http://purl.org/au-research/grants/arc/CE140100008
http://purl.org/au-research/grants/arc/CE1101007
Appears in Collections:Agriculture, Food and Wine publications

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.