Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/99635
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dc.contributor.authorGuy, M.en
dc.contributor.authorShaw, M.en
dc.contributor.authorWeiner, C.en
dc.contributor.authorHobson, L.en
dc.contributor.authorStark, Z.en
dc.contributor.authorRose, K.en
dc.contributor.authorKalscheuer, V.en
dc.contributor.authorGecz, J.en
dc.contributor.authorPhizicky, E.en
dc.date.issued2015en
dc.identifier.citationHuman Mutation, 2015; 36(12):1176-1187en
dc.identifier.issn1059-7794en
dc.identifier.issn1098-1004en
dc.identifier.urihttp://hdl.handle.net/2440/99635-
dc.description.abstracttRNA modifications are crucial for efficient and accurate protein synthesis, and modification defects are frequently associated with disease. Yeast trm7Δ mutants grow poorly due to lack of 2'-O-methylated C32 (Cm32 ) and Gm34 on tRNA(Phe), catalyzed by Trm7-Trm732 and Trm7-Trm734, respectively, which in turn results in loss of wybutosine at G37. Mutations in human FTSJ1, the likely TRM7 homolog, cause nonsyndromic X-linked intellectual disability (NSXLID), but the role of FTSJ1 in tRNA modification is unknown. Here, we report that tRNA(Phe) from two genetically independent cell lines of NSXLID patients with loss-of-function FTSJ1 mutations nearly completely lacks Cm32 and Gm34, and has reduced peroxywybutosine (o2yW37 ). Additionally, tRNA(Phe) from an NSXLID patient with a novel FTSJ1-p.A26P missense allele specifically lacks Gm34, but has normal levels of Cm32 and o2yW37. tRNA(Phe) from the corresponding Saccharomyces cerevisiae trm7-A26P mutant also specifically lacks Gm34, and the reduced Gm34 is not due to weaker Trm734 binding. These results directly link defective 2'-O-methylation of the tRNA anticodon loop to FTSJ1 mutations, suggest that the modification defects cause NSXLID, and may implicate Gm34 of tRNA(Phe) as the critical modification. These results also underscore the widespread conservation of the circuitry for Trm7-dependent anticodon loop modification of eukaryotic tRNA(Phe).en
dc.description.statementofresponsibilityMichael P. Guy, Marie Shaw, Catherine L. Weiner, Lynne Hobson, Zornitza Stark, Katherine Rose, Vera M. Kalscheuer, Jozef Gecz, and Eric M. Phizickyen
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.rights© 2015 WILEY PERIODICALS, INC.en
dc.subjectFTSJ1; intellectual disability; NSXLID; tRNA; 2′-O-methylation; TRM7en
dc.titleDefects in tRNA anticodon loop 2′-O-methylation are implicated in nonsyndromic X-linked intellectual disability due to mutations in FTSJ1en
dc.typeJournal articleen
dc.identifier.rmid0030034321en
dc.identifier.doi10.1002/humu.22897en
dc.identifier.pubid209467-
pubs.library.collectionMedicine publicationsen
pubs.library.teamDS06en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidShaw, M. [0000-0002-5060-190X]en
dc.identifier.orcidGecz, J. [0000-0002-7884-6861]en
Appears in Collections:Medicine publications

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