Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/99711
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Type: Journal article
Title: Microstructured optical fiber-based biosensors: reversible and nanoliter-scale measurement of zinc ions
Author: Heng, S.
McDevitt, C.
Kostecki, R.
Morey, J.
Eijkelkamp, B.
Ebendorff-Heidepriem, H.
Monro, T.
Abell, A.
Citation: ACS Applied Materials and Interfaces, 2016; 8(20):12727-12732
Publisher: American Chemical Society
Issue Date: 2016
ISSN: 1944-8244
1944-8252
Statement of
Responsibility: 
Sabrina Heng, Christopher A. McDevitt, Roman Kostecki, Jacqueline R. Morey, Bart A. Eijkelkamp, Heike Ebendorff-Heidepriem, Tanya M. Monro, and Andrew D. Abell
Abstract: Sensing platforms that allow rapid and efficient detection of metal ions would have applications in disease diagnosis and study, as well as environmental sensing. Here, we report the first microstructured optical fiber-based biosensor for the reversible and nanoliter-scale measurement of metal ions. Specifically, a photoswitchable spiropyran Zn(2+) sensor is incorporated within the microenvironment of a liposome attached to microstructured optical fibers (exposed-core and suspended-core microstructured optical fibers). Both fiber-based platforms retains high selectivity of ion binding associated with a small molecule sensor, while also allowing nanoliter volume sampling and on/off switching. We have demonstrated that multiple measurements can be made on a single sample without the need to change the sensor. The ability of the new sensing platform to sense Zn(2+) in pleural lavage and nasopharynx of mice was compared to that of established ion sensing methodologies such as inductively coupled plasma mass spectrometry (ICP-MS) and a commercially available fluorophore (Fluozin-3), where the optical-fiber-based sensor provides a significant advantage in that it allows the use of nanoliter (nL) sampling when compared to ICP-MS (mL) and FluoZin-3 (μL). This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modification and presents an opportunity for the development of new and highly specific ion sensors for real time sensing applications.
Keywords: biosensor; liposome; microstructured optical fiber; nanoscale; photoswitch; zinc
Description: Published: May 6, 2016
Rights: © 2016 American Chemical Society
RMID: 0030047736
DOI: 10.1021/acsami.6b03565
Grant ID: http://purl.org/au-research/grants/arc/CE140100003
http://purl.org/au-research/grants/arc/DP150101856
Appears in Collections:IPAS publications

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