Determination of Microcystin-LR in drinking water using UPLC tandem mass spectrometry-matrix effects and measurement
Date
2011
Authors
Li, W.
Duan, J.
Niu, C.
Qiang, N.
Mulcahy, D.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Chromatographic Science, 2011; 49(9):665-670
Statement of Responsibility
Conference Name
Abstract
A simple detection method using ultra-performance liquid chromatography electrospray ionisation tandem mass spectrometry (UPLC-ESI-MS-MS) coupled with the sample dilution method for determining trace microcystin-LR (MC-LR) in drinking water is presented. The limit of detection (LOD) was 0.04 ug/L and the limit of quantitation (LOQ) was 0.1 ug/L. Water matrix effects of ionic strength, dissolved organic carbon (DOC) and pH were examined. The results indicate that signal detection intensity for MC-LR was significantly suppressed as the ionic strength increased from ultrapure water condition, whereas it increased slightly with solution pH and DOC at low concentrations. However, addition of methanol (MeOH) into the sample was able to counter the signal suppression effects. In this study, dilution of the tap water sample by adding 4% MeOH (v/v) was observed to be adequate to compensate for the signal suppression. The recoveries of the samples fortified with MC-LR (0.2, 1, and 10 ug/L) for three different tap water samples ranged from 84.4% to 112.9%.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright 2011 Oxford University Press