Isolation, characterization and multiplex polymerase chain reaction of novel microsatellite loci for the avian parasite Philornis downsi (Diptera: Muscidae)
Date
2008
Authors
Dudaniec, R.
Gardner, M.
Kleindorfer, S.
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Journal article
Citation
Molecular Ecology Resources, 2008; 8(1):142-144
Statement of Responsibility
Rachael Y. Dudaniec, Michael G. Gardner, Sonia Kleindorfer
Conference Name
Abstract
An enrichment technique was used to isolate 11 di-, tri-, and tetra microsatellites for the parasitic fly Philornis downsi (Diptera: Muscidae). These loci were polymerase chain reaction amplified in singleplexes or two-plexes for P. downsi. The loci showed low to moderate polymorphism, exhibited between three and four alleles, and observed heterozygosity ranged from 0.05 to 0.86. These new markers will be useful for population-level and paternity analyses and will provide valuable information about the ecology of this high-impact parasite of vulnerable bird species.
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Dissertation Note
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