CaClOH-Modified Silica Nanoparticles for mRNA Delivery

Simple item page
dc.contributor.authorXian, H.
dc.contributor.authorSong, Y.
dc.contributor.authorQu, J.
dc.contributor.authorShi, Y.
dc.contributor.authorZhang, Y.
dc.contributor.authorWu, W.
dc.contributor.authorKim, M.
dc.contributor.authorWang, Y.
dc.contributor.authorYu, C.
dc.date.issued2025
dc.description.abstractMessenger RNA (mRNA) technology has attracted wide attention in biomedical applications; its success relies heavily on the development of effective delivery tools. Herein, we report the synthesis of a novel CaClOH-modified silica nanoparticle (SNP-CaClOH) with a spiky surface for mRNA delivery. SNP-CaClOH is obtained by a rationally designed thermal decomposition process of hydrated CaCl₂ inside the silanol-rich mesopores of preformed spiky SNPs. When used as a carrier for the cellular delivery of mRNA, the unique composition of CaClOH offers alkalinity to SNP-CaClOH that promotes endosomal escape via the proton sponge effect. Moreover, SNP-CaClOH leads to an increased intracellular Ca²⁺ level to activate the mammalian target of rapamycin complex 1 (mTORC1) by interacting with calmodulin (CaM) for enhanced mRNA translation. Taking further advantage of the spiky nanotopography, the superior mRNA delivery performance of SNP-CaClOH is demonstrated both in vitro and in vivo, providing useful delivery tools for mRNA technology development.
dc.description.statementofresponsibilityHe Xian, Yaping Song, Jingjing Qu, Yiru Shi, Yue Zhang, Weixi Wu, Minjun Kim, Yue Wang, Chengzhong Yu
dc.identifier.citationNano Letters, 2025; 25(16):6365-6373
dc.identifier.doi10.1021/acs.nanolett.4c05615
dc.identifier.issn1530-6984
dc.identifier.issn1530-6992
dc.identifier.orcidShi, Y. [0000-0001-9734-6574]
dc.identifier.urihttps://hdl.handle.net/2440/147813
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/DP200102962
dc.relation.granthttp://purl.org/au-research/grants/arc/LP22020093
dc.rights© 2025 American Chemical Society.
dc.source.urihttps://doi.org/10.1021/acs.nanolett.4c05615
dc.subjectmRNA delivery; CaClOH; silica nanoparticles; endosomal escape; mRNA translation
dc.titleCaClOH-Modified Silica Nanoparticles for mRNA Delivery
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Research Outputs