Blocking PI3K/AKT signaling inhibits bone sclerosis in subchondral bone and attenuates post-traumatic osteoarthritis
Date
2018
Authors
Lin, C.
Shao, Y.
Zeng, C.
Zhao, C.
Fang, H.
Wang, L.
Pan, J.
Liu, L.
Qi, W.
Feng, X.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Cellular Physiology, 2018; 233(8):6135-6147
Statement of Responsibility
Chuangxin Lin, Yan Shao, Chun Zeng, Chang Zhao, Hang Fang, Liping Wang, Jianying Pan, Liangliang Liu, Weizhong Qi, Xiaofeng Feng, Hong Qiu, Haiyang Zhang, Yuhui Chen, Hong Wang, Daozhang Cai, Cory J. Xian
Conference Name
Abstract
PI3K/AKT signaling is essential in regulating pathophysiology of osteoarthritis (OA). However, its potential modulatory role in early OA progression has not been investigated yet. Here, a mouse destabilization OA model in the tibia was used to investigate roles of PI3K/AKT signaling in the early subchondral bone changes and OA pathological process. We revealed a significant increase in PI3K/AKT signaling activation which was associated with aberrant bone formation in tibial subchondral bone following destabilizing the medial meniscus (DMM), which was effectively prevented by treatment with PI3K/AKT signaling inhibitor LY294002. PI3K/AKT signaling inhibition attenuated articular cartilage degeneration. Serum and bone biochemical analyses revealed increased levels of MMP-13, which was found expressed mainly by osteoblastic cells in subchondral bone. However, this MMP-13 induction was attenuated by LY294002 treatment. Furthermore, PI3K/AKT signaling was found to enhance preosteoblast proliferation, differentiation, and expression of MMP-13 by activating NF-κB pathway. In conclusion, inhibition of PI3K/AKT/NF-κB axis was able to prevent aberrant bone formation and attenuate cartilage degeneration in OA mice.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© 2018 Wiley Periodicals, Inc.