Host-defence skin peptides of the Australian common froglet Crinia signifera: sequence determination using positive and negative ion electrospray mass spectra
Date
2004
Authors
Maselli, V.
Brinkworth, C.
Bowie, J.
Tyler, M.
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Advisors
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Journal article
Citation
Rapid Communications in Mass Spectrometry, 2004; 18(18):2155-2161
Statement of Responsibility
Vita M. Maselli, Craig S. Brinkworth, John H. Bowie, Michael J. Tyler
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DOI
Abstract
The amino acid sequences of seven signiferin peptides are provided by consideration of tandem mass spectrometric (MS/MS) data for the respective MH+ and [M--H]- precursor ions. These methods do not differentiate between isomeric residues Leu and Ile; these were identified using the Edman degradation technique. The sequence of signiferin 1, a new smooth muscle contracting neuropeptide (RLCIPYIIPC-OH) containing a disulfide bridge, is best determined using the collision-induced dissociation (CID) spectrum of the [M--H]- anion. The initial fragmentation of this system involves loss of H2S2, which furnishes an open-chain system that is readily sequenced using the alpha and beta backbone cleavage anions.
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Dissertation Note
Provenance
Published Online: 13 Aug 2004
Description
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