Size analysis of poly(3-hydroxybutyrate) granules produced in recombinant Escherichia coli
Date
1995
Authors
Middelberg, A.
Lee, S.
Martin, J.
Williams, D.
Chang, H.
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Journal article
Citation
Biotechnology Letters, 1995; 17(2):205-210
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Abstract
Size distributions of PHB granules synthesized in recombinant Escherichia coli are determined by photosedimentation. Mean granule Stokes diameters are in the range 1.13-1.25 μm, which is larger than reported values for wild type microorganisms. Treatment with 1.5% hypochlorite and mild centrifugation did not affect granule size distribution. Treatment with 10% hypochlorite led to a significant reduction in mean diameter and total PHB. © 1995 Chapman & Hall.