Non-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo

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dc.contributor.authorDonnelley, M.
dc.contributor.authorMorgan, K.
dc.contributor.authorSiu, K.
dc.contributor.authorFarrow, N.
dc.contributor.authorStahr, C.
dc.contributor.authorBoucher, R.
dc.contributor.authorFouras, A.
dc.contributor.authorParsons, D.
dc.date.issued2014
dc.description.abstractTo determine the efficacy of potential cystic fibrosis (CF) therapies we have developed a novel mucociliary transit (MCT) measurement that uses synchrotron phase contrast X-ray imaging (PCXI) to non-invasively measure the transit rate of individual micron-sized particles deposited into the airways of live mice. The aim of this study was to image changes in MCT produced by a rehydrating treatment based on hypertonic saline (HS), a current CF clinical treatment. Live mice received HS containing a long acting epithelial sodium channel blocker (P308); isotonic saline; or no treatment, using a nebuliser integrated within a small-animal ventilator circuit. Marker particle motion was tracked for 20 minutes using PCXI. There were statistically significant increases in MCT in the isotonic and HS-P308 groups. The ability to quantify in vivo changes in MCT may have utility in pre-clinical research studies designed to bring new genetic and pharmaceutical treatments for respiratory diseases into clinical trials.
dc.description.statementofresponsibilityMartin Donnelley, Kaye S. Morgan, Karen K. W. Siu, Nigel R. Farrow, Charlene S. Stahr, Richard C. Boucher, Andreas Fouras & David W. Parsons
dc.identifier.citationScientific Reports, 2014; 4(3689):1-7
dc.identifier.doi10.1038/srep03689
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidDonnelley, M. [0000-0002-5320-7756]
dc.identifier.orcidFarrow, N. [0000-0002-2289-1268]
dc.identifier.orcidParsons, D. [0000-0002-8775-3501] [0000-0003-1746-3290]
dc.identifier.urihttp://hdl.handle.net/2440/81839
dc.language.isoen
dc.publisherNature Publishing Group
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/ © The Authors
dc.source.urihttps://doi.org/10.1038/srep03689
dc.subjectBiomedical engineering
dc.subjectPre-clinical studies
dc.subjectAnatomy
dc.titleNon-invasive airway health assessment: Synchrotron imaging reveals effects of rehydrating treatments on mucociliary transit in-vivo
dc.typeJournal article
pubs.publication-statusPublished

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