Enterococcus faecalis V583 cell membrane protein expression to alkaline stress.
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(Published version)
Date
2022
Authors
Cathro, P.
McCarthy, P.
Hoffmann, P.
Kidd, S.
Zilm, P.
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FEMS Microbiology Letters, 2022; 369(1):fnac082-1-fnac082-5
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Peter Cathro, Peter McCarthy, Peter Hoffmann, Stephen Kidd, Peter Zilm
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Abstract
Enterococcus faecalis is able to adapt to alkaline conditions and is commonly recovered from teeth in which endodontic treatment has failed. The role that E. faecalis membrane proteins play in survival strategies to extreme alkaline conditions is unclear. We grew E. faecalis V583 in a chemostat at pH 8 and 11 at one-tenth the organism’s relative maximumgrowth rate. Following membrane shaving, isotope-coding protein labels were added at the peptide level to samples and then combined. The relative proportion of membrane proteinswere identified using LC-ESImass spectrometry and MaxQuant analysis. Ratios ofmembrane proteinswere log2 transformed, with proteins deviating by more than 1 SD of the mean considered to be up- or down-regulated. A total of six proteins were upregulated in pH 11 including: EF0669 (polysaccharide biosynthesis family); EF1927 (glycerol uptake facilitator), and EF0114 (glycosyl hydrolase). A total of five proteins were down-regulated including: EF0108 (C4-dicarboxylate transporter); EF1838 (PTS system IIC component); EF0456 (PTS system IID component); and EF0022 (PTS mannose-specific IID component). In extreme alkaline conditions, themembrane proteins of E. faecalis seem to be involved in a shift of carbohydrate metabolism fromthe PTS system to glycerol, which supports the formation of a protective capsule protecting the cell.
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Data source: Supplementary data, https://doi.org/10.1093/femsle/fnac082
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© The Author(s) 2021. Published by Oxford University Press on behalf of FEMS. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact e-mail: journals.permissions@oup.com