Systematic evolution of ligands by exponential enrichment: photoselection of nucleic acid ligands and solution selex
Date
1999
Authors
Gold, L.
Willis, M.
Koch, T.
Ringquist, S.
Jensen, K.
Atkinson, B.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Patent
Citation
Statement of Responsibility
Inventors: Larry Gold, Michael Willis, Tad Koch, Steven Ringquist, Kirk Jensen, Brent Atkinson. Assignee: NeXstar Pharmaceuticals, Inc. Agents: Swanson & Bratschun LLC
Conference Name
Abstract
A method for identifying nucleic acid ligands to target molecules using the SELEX procedure wherein the candidate nucleic acids contain photoreactive groups and nucleic acid ligands identified thereby are claimed. The complexes of increased affinity nucleic acids and target molecules formed in the procedure are crosslinked by irradiation to facilitate separation from unbound nucleic acids. In other methods partitioning of high and low affinity nucleic acids is facilitated by primer extension steps as shown in the figure in which chain termination nucleotides, digestion resistant nucleotides or nucleotides that allow retention of the cDNA product on an affinity matrix arc differentially incorporated into the cDNA products of either the high or low affinity nucleic acids and the cDNA products are treated accordingly to amplification, enzymatic or chemical digestion or by contact with an affinity matrix.
School/Discipline
Dissertation Note
Provenance
Description
Publication number US6001577 A
Publication type Grant
Application number US 09/093,293
Publication date Dec 14, 1999
Filing date Jun 8, 1998
Priority date Jun 8, 1998