Structures of human primosome elongation complexes.
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(Accepted version)
Date
2023
Authors
He, Q.
Baranovskiy, A.G.
Morstadt, L.M.
Lisova, A.E.
Babayeva, N.D.
Lusk, B.L.
Lim, C.J.
Tahirov, T.H.
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Journal article
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Nature Structural & Molecular Biology, 2023; 30(5):579-583
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Qixiang He, Andrey G. Baranovskiy, Lucia M. Morstadt, Alisa E. Lisova, Nigar D. Babayeva, Benjamin L. Lusk, Ci Ji Lim, Tahir H. Tahirov
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Abstract
The synthesis of RNA–DNA primer by primosome requires coordination between primase and DNA polymerase α subunits, which is accompanied by unknown architectural rearrangements of multiple domains. Using cryogenic electron microscopy, we solved a 3.6 Å human primosome structure caught at an early stage of RNA primer elongation with deoxynucleotides. The structure confirms a long-standing role of primase large subunit and reveals new insights into how primosome is limited to synthesizing short RNA–DNA primers.
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Copyright © 2023, The Author(s), under exclusive licence to Springer Nature America, Inc.