SUR7 deletion in Candida albicans impacts extracellular vesicle features and delivery of virulence factors
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(Published version)
Date
2023
Authors
McKenna, J.A.
Garcia-Ceron, D.
Bleackley, M.R.
Yu, L.
Bulone, V.
Anderson, M.A.
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Journal article
Citation
Journal of Extracellular Biology, 2023; 2(5):e82-1-e82-18
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James A.McKenna, Donovan Garcia-Ceron, Mark R. Bleackley, Long Yu, Vincent Bulone, Marilyn A. Anderson
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Abstract
Extracellular vesicles (EVs) from human fungal pathogens have been implicated in fungal virulence, yet little is known about their role in the host-pathogen interaction. Progress has been hampered by the lack of a specific marker for fungal EVs that can be used to monitor EV isolation and tracking in biological systems. Here we report the effect of a SUR7 gene knockout on the production, properties, and role of EVs in the virulence of Candida albicans. Sur7 is a component of the membrane compartment of Can1 (MCC) complex and is enriched in the EVs from C. albicans and other fungal species. MCC is a plasma membrane complex which together with the eisosome, a cytoplasmic protein complex, is a key regulator in plasma membrane organisation and plasma membrane associated processes. The SUR7 knockout strain produces smaller EVs than the wild-type (WT) with different protein and carbohydrate cargos. Furthermore, proteins with known roles in Candida pathogenesis were present in WT EVs and absent or diminished in the sur7Δ EVs. We demonstrate that the reduced virulence of the sur7Δ cells can be partially restored with EVs from a WT strain. These findings demonstrate the importance of Sur7-like proteins in the biogenesis of EVs in fungi and enhance our understanding of the role of fungal EVs in human pathogenesis.
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This article has Corrections in Volume 4 Issue 7 Journal of Extracellular Biology, First Published online: July 24, 2025. The data availability statement for this article was missing. The below data availability statement has been added to the article: Data available on request from the authors, raw mass spectrometry data is made available as a supplementary file.
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© 2023 The Authors. Journal of Extracellular Biology published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.