Structural elucidation of rapid solution-mediated phase transitions in pharmaceutical solids using in situ synchrotron SAXS/WAXS

Date

2012

Authors

Boetker, J.
Rades, T.
Rantanen, J.
Hawley, A.
Boyd, B.J.

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Journal article

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Molecular Pharmaceutics, 2012; 9(9):2787-2791

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Johan Boetker, Thomas Rades, Jukka Rantanen, Adrian Hawley and Ben J. Boyd

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Abstract

In situ elucidation of kinetics of solution-mediated phase transformations using direct structural determination has been achieved using synchrotron SAXS/WAXS radiation. Using theophylline as a model drug with known phase transformation from anhydrate to monohydrate form in aqueous conditions within a few minutes, the kinetics of the structural transition were resolved at the second scale, and the results achieved agreed well with those determined using indirect approaches such as Raman spectroscopy. The recrystallization of the monohydrate in situ (due to its lower solubility) from dissolved anhydrate solution (higher solubility) is demonstrated directly, highlighting a major issue for such compounds in application. The technique has the additional benefit of having the potential to identify intermediate structures which are not readily achievable with in situ spectroscopic techniques, as well as being amenable to high throughput approaches.

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© 2012 American Chemical Society

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