Evaporative membrane modulation for comprehensive two-dimensional liquid chromatography
Date
2018
Authors
Fornells, E.
Barnett, B.
Bailey, M.
Hilder, E.F.
Shellie, R.A.
Breadmore, M.C.
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Journal article
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Analytica Chimica Acta, 2018; 1000:303-309
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Abstract
An evaporative membrane modulator was developed, built and evaluated to avoid loss of performance in the second dimension when coupling two-dimensional liquid chromatography systems. The automated interface reduces the volume after 1 D elution on-line by a pre-determined factor, regardless of the separation gradient. This volume reduction ensures that the injection volume in the 2 D is appropriate for the second column, avoiding the detrimental effects of overloading. In addition, the fraction solvent composition is constant over the length of the separation increasing reproducibility of 2 D separations. The evaporative membrane modulator was demonstrated with a 10-fold reduction, reducing the injection volume from 50 to 5 μL.
A consequence of the EMM device is a reduction in the capacity of the first dimension, which is decreased by a factor of 2.4, but the peak width at half maximum was reduced by up to 22% in the second dimension. When band broadening is considered, the corrected peak capacity with the modulator was only 10% lower than that without the modulator, but with a gain in peak height of 2-3, and a decrease in retention time between subsequent peak-slices reduced from 4s to be negligible. This improves peak shape and shows potential to facilitate peak identification and quantification in more complex applications.
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Data source: Figures & tables, https://doi.org/10.1016/j.aca.2017.11.053
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Copyright 2017 Elsevier