Multimodal imaging needle combining optical coherence tomography and fluorescence for imaging of live breast cancer cells labeled with a fluorescent analog of tamoxifen

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Date

2022

Authors

Scolaro, L.
Lorenser, D.
Quirk, B.C.
Kirk, R.W.
Ho, L.A.
Thomas, E.
Li, J.
Saunders, C.M.
Sampson, D.D.
Fuller, R.O.

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Journal article

Citation

Journal of Biomedical Optics, 2022; 27(7):076004-1-076004-15

Statement of Responsibility

Loretta Scolaro, Dirk Lorenser, Bryden C. Quirk, Rodney W. Kirk, Louisa A. Ho, Elizabeth Thomas, Jiawen Li, Christobel M. Saunders, David D. Sampson, Rebecca O. Fuller and Robert A. McLaughlin

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DOI

10.1117/1.jbo.27.7.076004

Abstract

Significance: Imaging needles consist of highly miniaturized focusing optics encased within a hypodermic needle. The needles may be inserted tens of millimeters into tissue and have the potential to visualize diseased cells well beyond the penetration depth of optical techniques applied externally. Multimodal imaging needles acquire multiple types of optical signals to differentiate cell types. However, their use has not previously been demonstrated with live cells. Aim: We demonstrate the ability of a multimodal imaging needle to differentiate cell types through simultaneous optical coherence tomography (OCT) and fluorescence imaging. Approach: We characterize the performance of a multimodal imaging needle. This is paired with a fluorescent analog of the therapeutic drug, tamoxifen, which enables cell-specific fluorescent labeling of estrogen receptor-positive (ER+) breast cancer cells. We perform simultaneous OCTand fluorescence in situ imaging on MCF-7 ER+ breast cancer cells and MDA-MB-231 ER− cells. Images are compared against unlabeled control samples and correlated with standard confocal microscopy images. Results:We establish the feasibility of imaging live cells with these miniaturized imaging probes by showing clear differentiation between cancerous cells. Conclusions: Imaging needles have the potential to aid in the detection of specific cancer cells within solid tissue.

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© The Authors. Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.27.7.076004]

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http://purl.org/au-research/grants/arc/DE180100112
 http://purl.org/au-research/grants/nhmrc/2001646
 http://purl.org/au-research/grants/nhmrc/GNT2008462
 http://purl.org/au-research/grants/arc/DP160104969
 http://purl.org/au-research/grants/arc/CE140100003
 http://purl.org/au-research/grants/arc/DP150104660
 http://purl.org/au-research/grants/nhmrc/1178912
 http://purl.org/au-research/grants/nhmrc/2001646
 http://purl.org/au-research/grants/nhmrc/2002254

Published Version

https://doi.org/10.1117/1.jbo.27.7.076004

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Persistent link to this record

https://hdl.handle.net/2440/136275