A set of 204 SNP and INDEL markers for Bigleaf maple (Acer macrophyllum Pursch)
Date
2015
Authors
Jardine, D.
Dormontt, E.
van Dijk, K.
Dixon, R.
Dunker, B.
Lowe, A.
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Journal article
Citation
Conservation Genetics Resources, 2015; 7(4):797-801
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D. I. Jardine, E. E. Dormontt, K.-J. van Dijk, R. R. M. Dixon, B. Dunker, A. J. Lowe
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Abstract
Novel single nucleotide polymorphisms (SNPs) and insertion/deletions (INDELs) were identified for the Bigleaf maple (Acer macrophyllum Pursch) using a combined next generation sequencing approach on the Ion Torrent PGM system with genotyping on the MassARRAY® iPLEX™ platform. Five hundred and ninety-eight putative loci were identified through sequencing of DNA fragments following a double restriction enzyme digest method. Two hundred and four polymorphic loci (199 SNPs and five INDELs) were successfully amplified across 65 individuals from seven populations across the native range of the species. Twenty-nine loci showed evidence of deviation from Hardy–Weinberg Equilibrium, and 85 were significantly linked. Expected heterozygosity ranged from 0.015 to 0.472 and FST from 0.011 to 0.359. These genetic resources will prove useful for future studies into the population genetics and phylogeography of this important and iconic timber species.
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© Springer Science+Business Media Dordrecht 2015