GABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters

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dc.contributor.authorRamesh, S.
dc.contributor.authorTyerman, S.
dc.contributor.authorXu, B.
dc.contributor.authorBose, J.
dc.contributor.authorKaur, S.
dc.contributor.authorConn, V.
dc.contributor.authorDomingos, P.
dc.contributor.authorUllah, S.
dc.contributor.authorWege, S.
dc.contributor.authorShabala, S.
dc.contributor.authorFeijó, J.
dc.contributor.authorRyan, P.
dc.contributor.authorGillham, M.
dc.date.issued2015
dc.description.abstractThe non-protein amino acid, gamma-aminobutyric acid (GABA) rapidly accumulates in plant tissues in response to biotic and abiotic stress, and regulates plant growth. Until now it was not known whether GABA exerts its effects in plants through the regulation of carbon metabolism or via an unidentified signalling pathway. Here, we demonstrate that anion flux through plant aluminium-activated malate transporter (ALMT) proteins is activated by anions and negatively regulated by GABA. Site-directed mutagenesis of selected amino acids within ALMT proteins abolishes GABA efficacy but does not alter other transport properties. GABA modulation of ALMT activity results in altered root growth and altered root tolerance to alkaline pH, acid pH and aluminium ions. We propose that GABA exerts its multiple physiological effects in plants via ALMT, including the regulation of pollen tube and root growth, and that GABA can finally be considered a legitimate signalling molecule in both the plant and animal kingdoms.
dc.description.statementofresponsibilitySunita A. Ramesh, Stephen D. Tyerman, Bo Xu, Jayakumar Bose, Satwinder Kaur, Vanessa Conn, Patricia Domingos, Sana Ullah, Stefanie Wege, Sergey Shabala, José A. Feijó, Peter R. Ryan & Matthew Gillham
dc.identifier.citationNature Communications, 2015; 6(1):7879-1-7879-9
dc.identifier.doi10.1038/ncomms8879
dc.identifier.issn2041-1723
dc.identifier.issn2041-1723
dc.identifier.orcidRamesh, S. [0000-0003-2230-4737]
dc.identifier.orcidTyerman, S. [0000-0003-2455-1643]
dc.identifier.orcidXu, B. [0000-0002-7583-2384]
dc.identifier.orcidBose, J. [0000-0002-0565-2951]
dc.identifier.orcidWege, S. [0000-0002-7232-5889]
dc.identifier.urihttp://hdl.handle.net/2440/93933
dc.language.isoen
dc.publisherNature Publishing Group
dc.relation.granthttp://purl.org/au-research/grants/arc/FT130100709
dc.relation.granthttp://purl.org/au-research/grants/arc/DP130104205
dc.rights© 2015 Macmillan Publishers Limited. All rights reserved. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.source.urihttps://doi.org/10.1038/ncomms8879
dc.subjectOocytes
dc.subjectAnimals
dc.subjectXenopus laevis
dc.subjectArabidopsis
dc.subjectHordeum
dc.subjectTriticum
dc.subjectVitis
dc.subjectAcidosis
dc.subjectAluminum
dc.subjectgamma-Aminobutyric Acid
dc.subjectBicuculline
dc.subjectMuscimol
dc.subjectOrganic Anion Transporters
dc.subjectPlant Proteins
dc.subjectMicroscopy, Confocal
dc.subjectPatch-Clamp Techniques
dc.subjectMutagenesis, Site-Directed
dc.subjectSignal Transduction
dc.subjectAmino Acid Motifs
dc.subjectMembrane Potentials
dc.subjectPollen Tube
dc.subjectStress, Physiological
dc.subjectGABA-A Receptor Agonists
dc.subjectGABA-A Receptor Antagonists
dc.subjectNicotiana
dc.titleGABA signalling modulates plant growth by directly regulating the activity of plant-specific anion transporters
dc.typeJournal article
pubs.publication-statusPublished

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