Single copy nuclear DNA markers for the onychophoran Phallocephale tallagandensis

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dc.contributor.authorSands, C.
dc.contributor.authorLancaster, M.
dc.contributor.authorAustin, J.
dc.contributor.authorSunnucks, P.
dc.date.issued2009
dc.description.abstractFor some species, particularly invertebrates, developing single copy nuclear markers is an expensive and time-consuming task that may result in few or no usable markers. This has proven true for Onychophora (velvet worms). Here we describe our PCR-based method of generating single copy nuclear markers in Onychophora—a phylum comprised of species generally regarded as rare and of high conservation value—for which suitable microsatellites have been difficult to obtain. We list 6 primer pairs, some of which amplify across genera, and demonstrate their utility in identifying strong population structure in the species Phallocephale tallagandensis.
dc.description.statementofresponsibilityChester J. Sands, Melanie L. Lancaster, Jeremy J. Austin, Paul Sunnucks
dc.identifier.citationConservation Genetics Resources, 2009; 1(1):17-19
dc.identifier.doi10.1007/s12686-009-9004-0
dc.identifier.issn1877-7252
dc.identifier.issn1877-7260
dc.identifier.orcidAustin, J. [0000-0003-4244-2942]
dc.identifier.urihttp://hdl.handle.net/2440/58797
dc.language.isoen
dc.publisherSpringer Netherlands
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0211156
dc.relation.granthttp://purl.org/au-research/grants/arc/DP0211156
dc.rights© Springer Science+Business Media B.V. 2009
dc.source.urihttps://doi.org/10.1007/s12686-009-9004-0
dc.subjectMarker development
dc.subjectAnonymous single copy nuclear markers
dc.subjectPopulation genetics
dc.subjectMicrosatellite
dc.subjectOnychophora
dc.titleSingle copy nuclear DNA markers for the onychophoran Phallocephale tallagandensis
dc.typeJournal article
pubs.publication-statusPublished

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