Simple method for the simultaneous demonstration of formation and resorption in undecalcified bone embedded in methyl methacrylate
Date
1997
Authors
McNeil, P.
Durbridge, T.
Parkinson, I.
Moore, R.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Journal of Histotechnology, 1997; 20(4):307-311
Statement of Responsibility
Peter J. McNeil, Timothy C. Durbridge, Ian H. Parkinson, Robert J. Moore
Conference Name
Abstract
A rapid method for processing mineralized bone biopsies with acrylic resin is described. The processing schedule is completed within 4 days, without the use of vacuum infiltration and using minimal amounts of reagent. Histological sections can be stained for selected features of bone, including acid phosphatase and alkaline phosphatase, in a combined histochemical procedure. This technique combines many favorable aspects from previously published methods into 1 reproducible procedure for the optimal preservation and demonstration of a number of histological entities in bone.