'TeeBot': A High Throughput Robotic Fermentation and Sampling System
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(Published version)
Date
2021
Authors
Van Holst Pellekaan, N.
Walker, M.
Watson, T.
Jiranek, V.
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Journal article
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Fermentation, 2021; 7(4):205-1-205-11
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Nicholas van Holst Pellekaan, Michelle E. Walker, Tommaso L. Watson and Vladimir Jiranek
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Abstract
When fermentation research requires the comparison of many strains or conditions, the major bottleneck is a technical one. Microplate approaches are not able to produce representative fermentative performance due to their inability to truly operate anaerobically, whilst more traditional methods do not facilitate sample density sufficient to assess enough candidates to be considered even medium throughput. Two robotic platforms have been developed that address these technological shortfalls. Both are built on commercially available liquid handling platforms fitted with custom labware. Results are presented detailing fermentation performance as compared to current best practice, i.e., shake flasks fitted with airlocks and sideports. The ‘TeeBot’ is capable sampling from 96 or 384 fermentations in 100 mL or 30 mL volumes, respectively, with airlock sealing and minimal headspace. Sampling and downstream analysis are facilitated by automated liquid handling, use of 96-well sample plate format and temporary cryo-storage (<0 °C).
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Published: 24 September 2021
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Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).