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The two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells

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dc.contributor.authorDorstyn, L.
dc.contributor.authorMills, K.
dc.contributor.authorLazebnik, Y.
dc.contributor.authorKumar, S.
dc.date.issued2004
dc.description.abstractIn Drosophila, activation of the apical caspase DRONC requires the apoptotic protease-activating factor homologue, DARK. However, unlike caspase activation in mammals, DRONC activation is not accompanied by the release of cytochrome c from mitochondria. Drosophila encodes two cytochrome c proteins, Cytc-p (DC4) the predominantly expressed species, and Cytc-d (DC3), which is implicated in caspase activation during spermatogenesis. Here, we report that silencing expression of either or both DC3 and DC4 had no effect on apoptosis or activation of DRONC and DRICE in Drosophila cells. We find that loss of function mutations in dc3 and dc4, do not affect caspase activation during Drosophila development and that ectopic expression of DC3 or DC4 in Drosophila cells does not induce caspase activation. In cell-free studies, recombinant DC3 or DC4 failed to activate caspases in Drosophila cell lysates, but remarkably induced caspase activation in extracts from human cells. Overall, our results argue that DARK-mediated DRONC activation occurs independently of cytochrome c.
dc.description.statementofresponsibilityLoretta Dorstyn, Kathryn Mills, Yuri Lazebnik, and Sharad Kumar
dc.identifier.citationJournal of Cell Biology, 2004; 167(3):405-410
dc.identifier.doi10.1083/jcb.200408054
dc.identifier.issn0021-9525
dc.identifier.issn1540-8140
dc.identifier.orcidKumar, S. [0000-0001-7126-9814]
dc.identifier.urihttp://hdl.handle.net/2440/9574
dc.language.isoen
dc.publisherRockefeller Univ Press
dc.source.urihttps://doi.org/10.1083/jcb.200408054
dc.subjectCell Line
dc.subjectAnimals
dc.subjectHumans
dc.subjectDrosophila melanogaster
dc.subjectCytochrome c Group
dc.subjectCytochromes c
dc.subjectCaspases
dc.subjectDrosophila Proteins
dc.subjectRecombinant Proteins
dc.subjectApoptosis
dc.subjectEnzyme Activation
dc.subjectProtein Binding
dc.subjectMutation
dc.titleThe two cytochrome c species, DC3 and DC4, are not required for caspase activation and apoptosis in Drosophila cells
dc.typeJournal article
pubs.publication-statusPublished

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