Unravelling structural dynamics within a photoswitchable single-peptide: a step towards multi-modal bio-inspired nanodevices

Date

2020

Authors

Chen, X.
Yeoh, Y.Q.
He, Y.
Zhou, C.
Horsley, J.R.
Abell, A.D.
Yu, J.
Guo, X.

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Journal article

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Angewandte Chemie International Edition, 2020; 59(50):22554-22562

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Xinjiani Chen, Yuan Qi Yeoh, Yanbin He, Chenguang Zhou, John R. Horsley, Andrew D. Abell, Jingxian Yu, and Xuefeng Guo

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Abstract

The majority of the protein structures have been elucidated under equilibrium conditions. The aim herein is to provide a better understanding of the dynamic behavior inherent to proteins by fabricating a label‐free nanodevice comprising a single‐peptide junction to measure real‐time conductance, from which their structural dynamic behavior can be inferred. This device contains an azobenzene photoswitch for interconversion between a well‐defined cis, and disordered trans isomer. Real‐time conductance measurements revealed three distinct states for each isomer, with molecular dynamics simulations showing each state corresponds to a specific range of hydrogen bond lengths within the cis isomer, and specific dihedral angles in the trans isomer. These insights into the structural dynamic behavior of peptides may rationally extend to proteins. Also demonstrated is the capacity to modulate conductance which advances the design and development of bioinspired electronic nanodevices.

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© 2020 Wiley-VCH GmbH

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